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round bottom suspension cell 96 well plate  (Greiner Bio)


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    Greiner Bio round bottom suspension cell 96 well plate
    Round Bottom Suspension Cell 96 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/suspension+cell+culture+96+well+plates/Suspension+Culture+Microplate+96+Well+Ps/us12590151-917-21-26
    Average 96 stars, based on 208 article reviews
    round bottom suspension cell 96 well plate - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Suspension:

    Article Title: K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. A day later, cells were harvested and plated into low-attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1000 MDA-MB-231 or 2500 MCF-7 cell were seeded per well of the 96-well plate in 50 μL of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v / v MethoCult (cat. no. SFH4636, Stemcell technologies, Grenoble, France), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/mL EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/mL FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: K-Ras Binds Calmodulin-Related centrin1 With Potential Implications for K-Ras Driven Cancer Cell Stemness
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. The next day, cells were collected by trypsinization and re-plated 12 of 16 into low attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1,000 MDA-MB-231 or 2,500 MCF-7 cell were seeded per well of the 96-well plate in 50 μl of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v/v MethoCult (cat. no. SFH4636, Stemcell technologies), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/ml EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/ml FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness.
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. A day later, cells were harvested and plated into low-attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1000 MDA-MB-231 or 2500 MCF-7 cell were seeded per well of the 96-well plate in 50 μL of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v/v MethoCult (cat. no. SFH4636, Stemcell technologies, Grenoble, France), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/mL EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/mL FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: Identification of an H-Ras nanocluster disrupting peptide
    Article Snippet: .. The cells were seeded in low attachment, suspension cell culture 96-well plates (Greiner, #655185). ..

    Article Title: Identification of an H-Ras nanocluster disrupting peptide
    Article Snippet: .. The cells were seeded in low attachment, suspension cell culture 96-well plates (Greiner, #655185). ..

    Cell Culture:

    Article Title: K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. A day later, cells were harvested and plated into low-attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1000 MDA-MB-231 or 2500 MCF-7 cell were seeded per well of the 96-well plate in 50 μL of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v / v MethoCult (cat. no. SFH4636, Stemcell technologies, Grenoble, France), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/mL EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/mL FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: K-Ras Binds Calmodulin-Related centrin1 With Potential Implications for K-Ras Driven Cancer Cell Stemness
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. The next day, cells were collected by trypsinization and re-plated 12 of 16 into low attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1,000 MDA-MB-231 or 2,500 MCF-7 cell were seeded per well of the 96-well plate in 50 μl of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v/v MethoCult (cat. no. SFH4636, Stemcell technologies), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/ml EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/ml FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness.
    Article Snippet: MDA-MB-231 and MCF-7 cells were seeded in 12-well plates (cat. No. 665180, Greiner Bio-One) and transfected with either 100 nM negative control siRNA or siRNA targeting CALM1 or CETN1 using Lipofectamine RNAiMAX. .. A day later, cells were harvested and plated into low-attachment, suspension cell culture 96-well plates (cat. no. 655185, Cellstar, Greiner Bio-One) for 3D spheroid suspension culture. .. About 1000 MDA-MB-231 or 2500 MCF-7 cell were seeded per well of the 96-well plate in 50 μL of RPMI medium (cat. no. 52400-025, Gibco, Thermo Fisher Scientific) or DMEM, respectively, containing 0.5% v/v MethoCult (cat. no. SFH4636, Stemcell technologies, Grenoble, France), 1x B27 (cat. no. 17504044, Gibco, Thermo Fisher Scientific), 25 ng/mL EGF (cat. no. E9644, Sigma-Aldrich), and 25 ng/mL FGF (cat. no. RP-8628, Thermo Fisher Scientific).

    Article Title: Identification of an H-Ras nanocluster disrupting peptide
    Article Snippet: .. The cells were seeded in low attachment, suspension cell culture 96-well plates (Greiner, #655185). ..

    Article Title: Identification of an H-Ras nanocluster disrupting peptide
    Article Snippet: .. The cells were seeded in low attachment, suspension cell culture 96-well plates (Greiner, #655185). ..



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